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Order Kazusa clone(s) from : |
| Product ID | ORK00109 |
|---|---|
| Accession No | AB014511 |
| Description | ATPase, class II, type 9A |
| Clone name | hg00483 |
| Vector information | |
| cDNA sequence | DNA sequence (7176 bp) Predicted protein sequence (912 aa) |
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HaloTag ORF Clone |
FHC00109
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| Flexi ORF Clone | FXC00109 |
| Source | Human adult brain |
| Rouge ID |
mKIAA0611
by Kazusa Mouse cDNA Project
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Length: 7176 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | Warning | No warning |
Length: 912 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| FPrintScan | IPR001757 | 254 | 268 | PR00119 | ATPase |
| IPR001757 | 534 | 544 | PR00119 | ATPase | |
| IPR001757 | 648 | 667 | PR00119 | ATPase | |
| HMMPfam | IPR005834 | 250 | 671 | PF00702 | Haloacid dehalogenase-like hydrolase |
| HMMTigr | IPR006539 | 39 | 912 | TIGR01652 | Phospholipid-translocating P-type ATPase |
| IPR001757 | 168 | 275 | TIGR01494 | ATPase | |
| IPR001757 | 504 | 561 | TIGR01494 | ATPase | |
| IPR001757 | 621 | 737 | TIGR01494 | ATPase | |
| ScanRegExp | IPR001757 | 256 | 262 | PS00154 | ATPase |
Prediction of transmembrane (TM) segments| Method | No. | N terminal | transmembrane region | C terminal | type | length | SOSUI2 | 1 | 61 | NQFKYFFNLYFLLLACSQFVPEM | 83 | SECONDARY | 23 | 2 | 90 | TYWVPLGFVLAVTVIREAVEEIR | 112 | SECONDARY | 23 | 3 | 162 | DLEVNCLTKILFGALVVVSLVMV | 184 | PRIMARY | 23 | 4 | 194 | YLQIIRFLLLFSNIIPISLRVNL | 216 | PRIMARY | 23 | 5 | 708 | SQFVIHRSLCISTMQAVFSSVFY | 730 | SECONDARY | 23 | 6 | 742 | IIGYSTIYTMFPVFSLVLDKDVK | 764 | SECONDARY | 23 | 7 | 787 | KTFLIWVLISIYQGSTIMYGALL | 809 | PRIMARY | 23 | 8 | 817 | HIVAISFTSLILTELLMVALTIQ | 839 | PRIMARY | 23 | 9 | 844 | LMTVAELLSLACYIASLVFLHEF | 866 | PRIMARY | 23 | 10 | 874 | TLSFLWKVSVITLVSCLPLYVLK | 896 | PRIMARY | 23 |
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RT-PCR
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RT-PCR-ELISA
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Experimental conditions| Primer_f | ATATGCTAATGCCTCCCCCAC |
|---|---|
| Primer_r | GTAGGATGGAGCAGAAGAATG |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 20
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | ATATGCTAATGCCTCCCCCAC |
| Primer_r | GTAGGATGGAGCAGAAGAATG |
| PCR product length | 161 bp |
| PCR conditions | 95 °C 15 sec 64 °C 60 sec 30 cycles |