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Order Kazusa clone(s) from : |
| Product ID | ORK01072 |
|---|---|
| Accession No | AB002354 |
| Description | pleckstrin homology domain containing, family M (with RUN domain) member 1, transcript variant 1 |
| Clone name | hh00006s1 |
| Vector information | |
| cDNA sequence | DNA sequence (5248 bp) Predicted protein sequence (1058 aa) |
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HaloTag ORF Clone |
FHC01072
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| Flexi ORF Clone | FXC01072 |
| Source | Human adult brain |
| Rouge ID |
mKIAA0356
by Kazusa Mouse cDNA Project
|
| Note | We replaced hh00006, former representative clones for KIAA0356 with hh00006s1. (2002/12/27) |
Length: 5248 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | No warning |
Length: 1058 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| HMMPfam | IPR004012 | 51 | 185 | PF02759 | RUN |
| IPR001849 | 537 | 627 | PF00169 | Pleckstrin-like | |
| HMMSmart | IPR004012 | 120 | 183 | SM00593 | RUN |
| IPR001849 | 537 | 629 | SM00233 | Pleckstrin-like | |
| IPR001849 | 686 | 781 | SM00233 | Pleckstrin-like | |
| IPR002219 | 989 | 1042 | SM00109 | Protein kinase C | |
| ProfileScan | IPR004012 | 43 | 185 | PS50826 | RUN |
| IPR001849 | 536 | 627 | PS50003 | Pleckstrin-like | |
| IPR002219 | 988 | 1042 | PS50081 | Protein kinase C |
RT-PCR
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|---|
Experimental conditions| Primer_f | ATTCAAAACTCACACGGCAGC |
|---|---|
| Primer_r | TGCATTGACTCTTCCCGCCTC |
| PCR conditions | 95 °C 30 sec 64 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 17
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | ATTCAAAACTCACACGGCAGC |
| Primer_r | TGCATTGACTCTTCCCGCCTC |
| PCR product length | 159 bp |
| PCR conditions | 95 °C 15 sec 64 °C 60 sec 30 cycles |