KIAA1054

Product ID	ORK07033
Accession No	AB028977
Description	synaptic vesicle glycoprotein 2C
Clone name	hh05240
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5962 bp) Predicted protein sequence (480 aa)
Source	Human adult brain
Rouge ID	mKIAA1054 by Kazusa Mouse cDNA Project

Length: 5962 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	Warning

Integrity of 3' end

Length of 3'UTR	4517 bp
Genome contig ID	gi51511721f_75441316
PolyA signal sequence (AGTAAA,-33)	+--------+--------+----*----+---- TAAGTAAAGCTTCTCTTGTTATCTGTAATGTAGAC
Flanking genome sequence (220331 - 220380)	----+--------+--------+--------+--------+----* AAAAAAAAAAAAAAACCTGTTAGTATATTCTGGATGTATTGTGTGTCCCT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	5	f	75526659	75661645	11	99.2	Perfect prediction

Length: 480 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q496J9	7.9e-206	100.0	Synaptic vesicl...	Homo sapiens
EAW95772	3.1e-205	99.6	synaptic vesicl...	Homo sapiens
AAI00825	4.2e-205	99.8	Synaptic vesicl...	Homo sapiens
XP_526889	1.8e-203	99.2	synaptic vesicl...	Pan troglodytes
XP_001105846	3.3e-203	99.0	similar to syna...	Macaca mulatta

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB018279	5.7e-148	64.2	KIAA0736
AB018278	3.6e-146	62.3	KIAA0735

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR005828	1	114	PF00083	General substrate transporter
HMMPfam	IPR011701	330	447	PF07690	Major facilitator superfamily MFS_1
HMMTigr	IPR005988	1	480	TIGR01299	Synaptic vesicle protein SV2
ProfileScan	IPR007114	1	475	PS50850	Major facilitator superfamily
ScanRegExp	IPR005829	3	28	PS00217	Sugar transporter superfamily

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	2	LLSGFGIGGAIPTVFSYFAEVLA	24	SECONDARY	23
	2	33	SWLCMFWMIGGIYASAMAWAIIP	55	PRIMARY	23
	3	70	HSWRVFVIVCALPCVSSVVALTF	92	PRIMARY	23
	4	334	WIYFVNFLGTLAVLPGNIVSALL	356	PRIMARY	23
	5	362	RLTMLGGSMVLSGISCFFLWFG	383	PRIMARY	22
	6	386	ESMMIGMLCLYNGLTISAWNSLD	408	SECONDARY	23
	7	423	GFGFLNALCKAAAVLGNLIFGSL	445	SECONDARY	23
	8	455	LLASTVLVCGGLVGLCLPDTRTQ	477	PRIMARY	23

Primer_f	GTCATTTCCAAAGCAGTCCAG
Primer_r	GAGCCTTTAGTGCATGAACAG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	GTCATTTCCAAAGCAGTCCAG
Primer_r	GAGCCTTTAGTGCATGAACAG
PCR product length	159 bp
PCR conditions	95 °C15 sec64 °C60 sec30 cycles