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Order Kazusa clone(s) from : |
| Product ID | ORK00587 |
|---|---|
| Accession No | AB014544 |
| Description | TLR4 interactor with leucine-rich repeats |
| Clone name | hj03618 |
| Vector information | |
| cDNA sequence | DNA sequence (4933 bp) Predicted protein sequence (887 aa) |
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HaloTag ORF Clone |
FHC00587
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| Flexi ORF Clone | FXC00587 |
| Source | Human adult brain |
| Rouge ID |
mKIAA0644
by Kazusa Mouse cDNA Project
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Length: 4933 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | No warning |
Length: 887 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| FPrintScan | IPR001611 | 161 | 174 | PR00019 | Leucine-rich repeat |
| IPR001611 | 376 | 389 | PR00019 | Leucine-rich repeat | |
| HMMPfam | IPR001611 | 160 | 182 | PF00560 | Leucine-rich repeat |
| IPR001611 | 184 | 206 | PF00560 | Leucine-rich repeat | |
| IPR001611 | 208 | 230 | PF00560 | Leucine-rich repeat | |
| IPR001611 | 232 | 254 | PF00560 | Leucine-rich repeat | |
| IPR001611 | 256 | 278 | PF00560 | Leucine-rich repeat | |
| IPR001611 | 306 | 328 | PF00560 | Leucine-rich repeat | |
| IPR001611 | 330 | 352 | PF00560 | Leucine-rich repeat | |
| IPR001611 | 354 | 376 | PF00560 | Leucine-rich repeat | |
| IPR001611 | 378 | 400 | PF00560 | Leucine-rich repeat | |
| IPR001611 | 402 | 424 | PF00560 | Leucine-rich repeat | |
| IPR000483 | 463 | 491 | PF01463 | Cysteine-rich flanking region | |
| HMMSmart | IPR003591 | 158 | 181 | SM00369 | Leucine-rich repeat |
| IPR003591 | 182 | 205 | SM00369 | Leucine-rich repeat | |
| IPR003591 | 206 | 229 | SM00369 | Leucine-rich repeat | |
| IPR003591 | 230 | 253 | SM00369 | Leucine-rich repeat | |
| IPR003591 | 254 | 277 | SM00369 | Leucine-rich repeat | |
| IPR003591 | 278 | 303 | SM00369 | Leucine-rich repeat | |
| IPR003591 | 304 | 327 | SM00369 | Leucine-rich repeat | |
| IPR003591 | 328 | 351 | SM00369 | Leucine-rich repeat | |
| IPR003591 | 352 | 375 | SM00369 | Leucine-rich repeat | |
| IPR003591 | 376 | 399 | SM00369 | Leucine-rich repeat | |
| IPR003591 | 400 | 423 | SM00369 | Leucine-rich repeat | |
| IPR000483 | 435 | 491 | SM00082 | Cysteine-rich flanking region |
Prediction of transmembrane (TM) segments| Method | No. | N terminal | transmembrane region | C terminal | type | length | SOSUI2 | 1 | 772 | QLLTLALLTVNALLVLLALAAWA | 794 | PRIMARY | 23 |
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RT-PCR
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RT-PCR-ELISA
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Experimental conditions| Primer_f | CCAGCAGGGTAATATTGAGTC |
|---|---|
| Primer_r | AAGTAGAACACAGATCCAGGC |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 7
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | CCAGCAGGGTAATATTGAGTC |
| Primer_r | AAGTAGAACACAGATCCAGGC |
| PCR product length | 123 bp |
| PCR conditions | 95 °C 15 sec 62 °C 60 sec 30 cycles |