KIAA1137

Product ID	ORK04226
Accession No	AB032963
Description	ATPase, aminophospholipid transporter, class I, type 8B, member 2
Clone name	hj03907
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4990 bp) Predicted protein sequence (933 aa)
Source	Human adult brain
Rouge ID	mKIAA1137 by Kazusa Mouse cDNA Project

Length: 4990 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	2186 bp
Genome contig ID	gi89161185f_152473624
PolyA signal sequence (TATAAA,-21)	+--------+--------+----*----+---- GAAAAATGCCTTTTTATAAAATTTCAATTTCTGAG
Flanking genome sequence (116783 - 116832)	----+--------+--------+--------+--------+----* AAGTGTGTAATTTGTCTCTTTTCTGATGTTTAACCAAGACTGGTGGTGAA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	1	f	152573624	152590405	18	99.4	Perfect prediction

Length: 933 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
EAW53208	99.9	ATPase, Class I...	Homo sapiens
P98198	99.9	Probable phosph...	Homo sapiens
XP_524888	99.9	ATPase, Class I...	Pan troglodytes
AAQ19027	99.9	possible aminop...	Homo sapiens
XP_001114383	99.5	ATPase, Class I...	Macaca mulatta

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB075819	0	67.9	KIAA1939
AB028944	1.4e-81	36.3	KIAA1021
AB040920	1e-72	35.7	KIAA1487
AB011138	5.7e-70	32.6	KIAA0566
AB018258	1.8e-69	32.0	KIAA0715

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001757	133	147	PR00119	ATPase
FPrintScan	IPR001757	555	574	PR00119	ATPase
HMMTigr	IPR006539	1	844	TIGR01652	Phospholipid-translocating P-type ATPase
	IPR001757	63	154	TIGR01494	ATPase
	IPR001757	385	434	TIGR01494	ATPase
	IPR001757	520	635	TIGR01494	ATPase
ScanRegExp	IPR001757	135	141	PS00154	ATPase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	16	MNTLVLWIFGFLVCMGVILAIGN	38	PRIMARY	23
	2	65	SGFLSFWSYIIILNTVVPISLYV	87	PRIMARY	23
	3	617	YFFYKNFAFTMVHFWFGFFCGFS	639	SECONDARY	23
	4	649	ITLYNIVYTSLPVLAMGVFDQDV	671	SECONDARY	23
	5	698	FICIAQGIYTSVLMFFIPYGVFA	720	SECONDARY	23
	6	733	YQSFAVTVATSLVIVVSVQIGLD	755	PRIMARY	23
	7	761	AINHFFIWGSLAVYFAILFAMHS	783	SECONDARY	23
	8	808	TVWLTIVLTTVVCIMPVVAFRFL	830	PRIMARY	23

Primer_f	AGCATCTATGAGGAGGTTGAG
Primer_r	AAACCTCAGTCATGTCATCCG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	unigene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-