KIAA0956

Product ID	ORK04211
Accession No	AB023173
Description	ATPase, class VI, type 11B
Clone name	hj05590
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5542 bp) Predicted protein sequence (672 aa)
Source	Human adult brain
Rouge ID	mKIAA0956 by Kazusa Mouse cDNA Project

Length: 5542 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	3521 bp
Genome contig ID	gi89161205f_183966820
PolyA signal sequence (AATAAA,-19)	+--------+--------+----*----+---- GAAGTTATATTTATCAAATAAAAACTTTCCTATAT
Flanking genome sequence (155291 - 155340)	----+--------+--------+--------+--------+----* AATTAAAAGTGTTCCTTTATTTAAGCATTTTAAGCCTGTCTCTGTTTATC

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	3	f	184066820	184122109	17	99.3	Perfect prediction

Length: 672 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAG65593	100.0	unnamed protein...	Homo sapiens
EAW78344	100.0	ATPase, Class V...	Homo sapiens
Q9Y2G3	100.0	Probable phosph...	Homo sapiens
EAW78345	99.0	ATPase, Class V...	Homo sapiens
BAG52754	99.8	unnamed protein...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB028944	2.4e-97	57.9	KIAA1021
AB014511	4.1e-52	33.4	KIAA0611
AB032963	4.5e-45	37.9	KIAA1137
AB075819	2.3e-44	37.7	KIAA1939
AB040920	1.4e-43	36.6	KIAA1487

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001757	193	203	PR00119	ATPase
FPrintScan	IPR001757	314	333	PR00119	ATPase
HMMTigr	IPR006539	1	602	TIGR01652	Phospholipid-translocating P-type ATPase
	IPR001757	168	218	TIGR01494	ATPase
	IPR001757	286	394	TIGR01494	ATPase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	25	DEKALVEAAARIGIVFIGNSEET	47	SECONDARY	23
	2	405	SVYLTLYNICFTSLPILIYSLLE	427	SECONDARY	23
	3	494	WTFGTLVFTVMVITVTVKMALET	516	SECONDARY	23
	4	527	TWGSIIFYFVFSLFYGGILWPFL	549	PRIMARY	23
	5	560	QLLSSGSAWFAIILMVVTCLFLD	582	PRIMARY	23

Primer_f	GAACTTATCTGATCGCTTGAG
Primer_r	CACGAGATTTTTCAGAGTAGC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	UniGene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-