KIAA0736

Product ID	ORK00618
Accession No	AB018279
Description	synaptic vesicle glycoprotein 2A, transcript variant 1
Clone name	hk03846
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4353 bp) Predicted protein sequence (748 aa)
HaloTag ORF Clone	FHC00618
Flexi ORF Clone	FXC00618
Source	Human adult brain
Rouge ID	mKIAA0736 by Kazusa Mouse cDNA Project

Length: 4353 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	1690 bp
Genome contig ID	gi89161185r_148041558
PolyA signal sequence (AATAAA,-27)	+--------+--------+----*----+---- TGACGAAAAATAAAACGGAAACAAATCCTCAAAAT
Flanking genome sequence (99942 - 99893)	----+--------+--------+--------+--------+----* ACAAAATGGCTATACCTGCTTCTGTGACTCTCCACCTACATCCAATTCTC

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	1	r	148141500	148156001	13	99.8	Perfect prediction

Length: 748 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q7L0J3	100.0	Synaptic vesicl...	Homo sapiens
XP_001166661	99.9	synaptic vesicl...	Pan troglodytes
BAF84407	99.9	unnamed protein...	Homo sapiens
CAD97824	99.7	hypothetical pr...	Homo sapiens
Q5R4L9	99.6	Synaptic vesicl...	Pongo abelii

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB018278	3e-140	62.4	KIAA0735
AB028977	1.9e-103	64.2	KIAA1054

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR011701	178	715	PF07690	Major facilitator superfamily MFS_1
HMMTigr	IPR005988	7	748	TIGR01299	Synaptic vesicle protein SV2
ProfileScan	IPR007114	174	743	PS50850	Major facilitator superfamily
ScanRegExp	IPR005829	270	295	PS00217	Sugar transporter superfamily

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	174	LYFVLGLALMADGVEVFVVGFVL	196	PRIMARY	23
	2	211	GMLGLIVYLGMMVGAFLWGGLAD	233	PRIMARY	23
	3	239	QCLLISLSVNSVFAFFSSFVQGY	261	SECONDARY	23
	4	266	FCRLLSGVGIGGSIPIVFSYFSE	288	PRIMARY	23
	5	301	WLCMFWMIGGVYAAAMAWAIIPH	323	SECONDARY	23
	6	337	HSWRVFVLVCAFPSVFAIGALTT	359	PRIMARY	23
	7	602	MVYFVSFLGTLAVLPGNIVSALL	624	PRIMARY	23
	8	632	RMLAGSSVMSCVSCFFLSF	650	SECONDARY	19
	9	659	ALLCLFGGVSIASWNALDVLTVE	681	SECONDARY	23
	10	691	AFGFLNALCKLAAVLGISIFTSF	713	SECONDARY	23
	11	723	LFASAALALGSSLALKLPETRGQ	745	SECONDARY	23

Primer_f	ATTCACCTTCGTCATGCTGCG
Primer_r	ACACTCATACACTGGGCACTG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	ATTCACCTTCGTCATGCTGCG
Primer_r	ACACTCATACACTGGGCACTG
PCR product length	119 bp
PCR conditions	95 °C15 sec62 °C60 sec30 cycles