FLJ00357

Product ID	ORK05172
Accession No	AK160383
Clone name	sh02980
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5614 bp) Predicted protein sequence (628 aa)
Source	Human spleen

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	Warning

Length: 628 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAD18726	100.0	FLJ00357 protei...	Homo sapiens
Q96P48	99.5	Arf-GAP, Rho-GA...	Homo sapiens
EAW74864	99.5	centaurin, delt...	Homo sapiens
AAI44563	99.3	ARAP1 protein [...	Homo sapiens
BAG09846	99.3	centaurin-delta...	synthetic construct

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB018325	1.1e-190	99.3	KIAA0782
AB011152	6.8e-71	46.4	KIAA0580
D79989	2e-11	30.2	KIAA0167
AB051503	2.4e-10	29.3	KIAA1716
AK160375	1.9e-09	22.9	FLJ00312

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR001849	117	208	PF00169	Pleckstrin-like
HMMPfam	IPR001164	324	448	PF01412	Human Rev interacting-like protein (hRIP)
HMMSmart	IPR001849	117	210	SM00233	Pleckstrin-like
	IPR001849	230	320	SM00233	Pleckstrin-like
	IPR001164	314	450	SM00105	Human Rev interacting-like protein (hRIP)
	IPR001849	533	628	SM00233	Pleckstrin-like
ProfileScan	IPR001849	116	208	PS50003	Pleckstrin-like
	IPR001849	229	318	PS50003	Pleckstrin-like
	IPR001164	324	449	PS50115	Human Rev interacting-like protein (hRIP)
	IPR001849	532	628	PS50003	Pleckstrin-like

Primer_f
Primer_r
PCR conditions	test