FLJ00312

Product ID	ORK04671
Accession No	AK160375
Clone name	sh06481
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5381 bp) Predicted protein sequence (663 aa)
Source	Human spleen

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	Warning

Length: 663 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAD18718	0	100.0	FLJ00312 protei...	Homo sapiens
XP_001716862	2.4e-172	96.9	similar to FLJ0...	Homo sapiens
Q5T2P9	9.9e-172	96.8	Centaurin-gamma...	Homo sapiens
XP_001714838	1e-171	96.8	similar to FLJ0...	Homo sapiens
NP_001071133	1.7e-166	92.6	centaurin, gamm...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB075855	1.3e-65	84.6	KIAA1975
AB029022	6.9e-49	71.5	KIAA1099
D79989	9.2e-29	42.0	KIAA0167
AB051503	2.6e-16	34.7	KIAA1716
D30758	2.8e-15	34.3	KIAA0050

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001164	481	500	PR00405	Human Rev interacting-like protein (hRIP)
	IPR001164	500	517	PR00405	Human Rev interacting-like protein (hRIP)
	IPR001164	521	542	PR00405	Human Rev interacting-like protein (hRIP)
HMMPfam	IPR001849	308	468	PF00169	Pleckstrin-like
HMMPfam	IPR001164	469	589	PF01412	Human Rev interacting-like protein (hRIP)
HMMSmart	IPR001849	308	470	SM00233	Pleckstrin-like
HMMSmart	IPR001164	469	589	SM00105	Human Rev interacting-like protein (hRIP)
ProfileScan	IPR001849	307	468	PS50003	Pleckstrin-like
	IPR001164	469	589	PS50115	Human Rev interacting-like protein (hRIP)
	IPR002110	596	648	PS50297	Ankyrin

Primer_f
Primer_r
PCR conditions	test