HUGE

Gene/Protein Characteristic Table for KIAA1250

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK05646
Accession No. :	AB033076
Description :	kinase D-interacting substrate of 220 kDa.
HUGO Gene Name :
Clone Name :	pf01137 [Vector Info]
Source :	Human brain (hippocampus)
Note :	We replaced hh06271, former representative clones for KIAA1250 with pf01137. (2001/5/29)

Features of the cloned DNA sequence	Description

Length: 7264 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 1791 bp

Genome contig ID gi89161199r_8686511
PolyA signal sequence
(AATAAA,-18)
+----*----+----*----+----*----+----
AAATAGATTTAGAAAACAATAAAAAATTGCATGCT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
ATTCTGACTCATGAATTTTTATTCACTATGATGATTCACATTTTGATTAA

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 2 r 8786511 8895181 30 99.6 Perfect prediction

Features of the protein sequence	Description

Length: 1777 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q9ULH0	100.0	Ankyrin repeat-...	Homo sapiens
XP_001083115	99.1	similar to kina...	Macaca mulatta
XP_532865	96.0	similar to kina...	Canis lupus fam...
XP_001083357	95.9	similar to kina...	Macaca mulatta
Q9EQG6	93.3	Ankyrin repeat-...	Rattus norvegicus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB014597	1.3e-18	32.1	KIAA0697
AB033049	1.5e-17	34.7	KIAA1223
AB002377	1.7e-14	28.6	KIAA0379
AB023174	5.9e-12	30.2	KIAA0957
AB051515	4.7e-10	30.5	KIAA1728

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

FPrintScan IPR002110 44 56 PR01415 Ankyrin

IPR002110 155 167 PR01415 Ankyrin

HMMPfam IPR002110 43 75 PF00023 Ankyrin

IPR002110 76 108 PF00023 Ankyrin

IPR002110 109 141 PF00023 Ankyrin

IPR002110 142 174 PF00023 Ankyrin

IPR002110 175 207 PF00023 Ankyrin

IPR002110 208 240 PF00023 Ankyrin

IPR002110 241 273 PF00023 Ankyrin

IPR002110 274 306 PF00023 Ankyrin

IPR002110 307 339 PF00023 Ankyrin

IPR002110 340 372 PF00023 Ankyrin

IPR002110 373 396 PF00023 Ankyrin

IPR011646 446 959 PF07693 KAP P-loop

HMMSmart IPR002110 43 72 SM00248 Ankyrin

IPR002110 76 105 SM00248 Ankyrin

IPR002110 109 138 SM00248 Ankyrin

IPR002110 142 171 SM00248 Ankyrin

IPR002110 175 204 SM00248 Ankyrin

IPR002110 208 237 SM00248 Ankyrin

IPR002110 241 270 SM00248 Ankyrin

IPR002110 274 303 SM00248 Ankyrin

IPR002110 307 336 SM00248 Ankyrin

IPR002110 340 369 SM00248 Ankyrin

IPR002110 373 404 SM00248 Ankyrin

ProfileScan IPR002110 23 396 PS50297 Ankyrin

IPR002110 43 75 PS50088 Ankyrin

IPR002110 76 108 PS50088 Ankyrin

IPR002110 109 141 PS50088 Ankyrin

IPR002110 142 174 PS50088 Ankyrin

IPR002110 175 207 PS50088 Ankyrin

IPR002110 208 240 PS50088 Ankyrin

IPR002110 241 273 PS50088 Ankyrin

IPR002110 274 306 PS50088 Ankyrin

IPR002110 340 372 PS50088 Ankyrin

IPR002110 373 396 PS50088 Ankyrin

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR002110	44	56	PR01415	Ankyrin
IPR002110	155	167	PR01415	Ankyrin
HMMPfam	IPR002110	43	75	PF00023	Ankyrin
IPR002110	76	108	PF00023	Ankyrin
IPR002110	109	141	PF00023	Ankyrin
IPR002110	142	174	PF00023	Ankyrin
IPR002110	175	207	PF00023	Ankyrin
IPR002110	208	240	PF00023	Ankyrin
IPR002110	241	273	PF00023	Ankyrin
IPR002110	274	306	PF00023	Ankyrin
IPR002110	307	339	PF00023	Ankyrin
IPR002110	340	372	PF00023	Ankyrin
IPR002110	373	396	PF00023	Ankyrin
IPR011646	446	959	PF07693	KAP P-loop
HMMSmart	IPR002110	43	72	SM00248	Ankyrin
IPR002110	76	105	SM00248	Ankyrin
IPR002110	109	138	SM00248	Ankyrin
IPR002110	142	171	SM00248	Ankyrin
IPR002110	175	204	SM00248	Ankyrin
IPR002110	208	237	SM00248	Ankyrin
IPR002110	241	270	SM00248	Ankyrin
IPR002110	274	303	SM00248	Ankyrin
IPR002110	307	336	SM00248	Ankyrin
IPR002110	340	369	SM00248	Ankyrin
IPR002110	373	404	SM00248	Ankyrin
ProfileScan	IPR002110	23	396	PS50297	Ankyrin
IPR002110	43	75	PS50088	Ankyrin
IPR002110	76	108	PS50088	Ankyrin
IPR002110	109	141	PS50088	Ankyrin
IPR002110	142	174	PS50088	Ankyrin
IPR002110	175	207	PS50088	Ankyrin
IPR002110	208	240	PS50088	Ankyrin
IPR002110	241	273	PS50088	Ankyrin
IPR002110	274	306	PS50088	Ankyrin
IPR002110	340	372	PS50088	Ankyrin
IPR002110	373	396	PS50088	Ankyrin

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 1 LQLSVKMSVLISQSVINYVEEEN 23 SECONDARY 23

2 502 QFSWLIVFLTLLLCGGLGLLFAF 524 PRIMARY 23

3 531 GIAVSLSFLALLYIFFIVIYFGG 553 PRIMARY 23

4 663 LPSFVIFLFIIGCIISGITLLAI 685 PRIMARY 23

5 693 LTVNAVLISIASVVGLAFVLNCR 715 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	1	LQLSVKMSVLISQSVINYVEEEN	23	SECONDARY	23
2	502	QFSWLIVFLTLLLCGGLGLLFAF	524	PRIMARY	23
3	531	GIAVSLSFLALLYIFFIVIYFGG	553	PRIMARY	23
4	663	LPSFVIFLFIIGCIISGITLLAI	685	PRIMARY	23
5	693	LTVNAVLISIASVVGLAFVLNCR	715	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: AGCCGAGTTAAGAGAATGGTC

Primer_r
: CCATCTACCGTCCTAACTGTG

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 2

Experimental conditions

Panel name	: unigene
Primer_f	: -
Primer_r	: -
PCR product length	: -
PCR conditions	: -

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	1791 bp
Genome contig ID	gi89161199r_8686511
PolyA signal sequence (AATAAA,-18)	+--------+--------+----*----+---- AAATAGATTTAGAAAACAATAAAAAATTGCATGCT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ATTCTGACTCATGAATTTTTATTCACTATGATGATTCACATTTTGATTAA

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	2	r	8786511	8895181	30	99.6	Perfect prediction

Primer_f	: AGCCGAGTTAAGAGAATGGTC
Primer_r	: CCATCTACCGTCCTAACTGTG
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1250

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information