HUGE

Gene/Protein Characteristic Table for KIAA1939

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Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK04227
Accession No. :	AB075819
Description :	Probable phospholipid-transporting ATPase IM.
HUGO Gene Name :	ATPase, class I, type 8B, member 4 (ATP8B4)
Clone Name :	ah01164 [Vector Info]
Source :	Human brain (amygdala)

Features of the cloned DNA sequence	Description

Length: 5782 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
YES

Integrity of 3' end

Length of 3'UTR 1954 bp

Genome contig ID gi51511731r_47837728
PolyA signal sequence
(AGTAAA,-24)
+----*----+----*----+----*----+----
TGAAAACTTTCAGTAAATGTTTTGGCACTATTGGT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
ACTTGATGTTTTATCTCTTCTTTATGGGAAATTCATTGGGGAGATTTTTG

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 15 r 47937728 48192805 29 99.5 Perfect prediction

Features of the protein sequence	Description

Length: 1082 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q8TF62	99.4	Probable phosph...	Homo sapiens
XP_510393	98.9	ATPase class I ...	Pan troglodytes
EAW77381	99.9	ATPase, Class I...	Homo sapiens
XP_001167879	99.3	ATPase class I ...	Pan troglodytes
XP_001499742	93.0	ATPase, class I...	Equus caballus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB032963	0	68.1	KIAA1137
AB028944	1.8e-80	34.8	KIAA1021
AB040920	5.5e-69	35.1	KIAA1487
AB018258	2.1e-65	31.3	KIAA0715
AB011138	5.6e-65	32.1	KIAA0566

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

FPrintScan IPR001757 280 294 PR00119 ATPase

IPR001757 703 722 PR00119 ATPase

HMMPfam IPR013200 700 720 PF08282 HAD superfamily hydrolase-like

HMMTigr IPR006539 2 992 TIGR01652 Phospholipid-translocating P-type ATPase

IPR001757 208 302 TIGR01494 ATPase

IPR001757 532 581 TIGR01494 ATPase

IPR001757 676 783 TIGR01494 ATPase

ScanRegExp IPR001757 282 288 PS00154 ATPase

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001757	280	294	PR00119	ATPase
IPR001757	703	722	PR00119	ATPase
HMMPfam	IPR013200	700	720	PF08282	HAD superfamily hydrolase-like
HMMTigr	IPR006539	2	992	TIGR01652	Phospholipid-translocating P-type ATPase
IPR001757	208	302	TIGR01494	ATPase
IPR001757	532	581	TIGR01494	ATPase
IPR001757	676	783	TIGR01494	ATPase
ScanRegExp	IPR001757	282	288	PS00154	ATPase

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 163 MNTLVLWIFGFLICLGIILAIGN 185 PRIMARY 23

2 214 FLTFWSYIIILNTVVPISLYVSV 236 PRIMARY 23

3 771 FAFTLVHFWFGFFCGFSAQTVYD 793 SECONDARY 23

4 797 ITLFNIVYTSLPVLAMGIFDQDV 819 SECONDARY 23

5 845 FFICVLHGIYTSLVLFFIPYGAF 867 PRIMARY 23

6 881 YQSFAVTMATSLVIVVSVQIALD 903 PRIMARY 23

7 908 TFINHVFIWGSIAIYFSILFTMH 930 PRIMARY 23

8 952 LTQKCIWLVILLTTVASVMPVVA 974 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	163	MNTLVLWIFGFLICLGIILAIGN	185	PRIMARY	23
2	214	FLTFWSYIIILNTVVPISLYVSV	236	PRIMARY	23
3	771	FAFTLVHFWFGFFCGFSAQTVYD	793	SECONDARY	23
4	797	ITLFNIVYTSLPVLAMGIFDQDV	819	SECONDARY	23
5	845	FFICVLHGIYTSLVLFFIPYGAF	867	PRIMARY	23
6	881	YQSFAVTMATSLVIVVSVQIALD	903	PRIMARY	23
7	908	TFINHVFIWGSIAIYFSILFTMH	930	PRIMARY	23
8	952	LTQKCIWLVILLTTVASVMPVVA	974	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: GTGCAGATAGCCTTGGATACC

Primer_r
: AGAGAATTACAAGCCAGATGC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 15

Experimental conditions

Panel name	: genbank
Primer_f	: -
Primer_r	: -
PCR product length	: -
PCR conditions	: -

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	YES

Length of 3'UTR	1954 bp
Genome contig ID	gi51511731r_47837728
PolyA signal sequence (AGTAAA,-24)	+--------+--------+----*----+---- TGAAAACTTTCAGTAAATGTTTTGGCACTATTGGT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ACTTGATGTTTTATCTCTTCTTTATGGGAAATTCATTGGGGAGATTTTTG

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	15	r	47937728	48192805	29	99.5	Perfect prediction

Primer_f	: GTGCAGATAGCCTTGGATACC
Primer_r	: AGAGAATTACAAGCCAGATGC
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1939

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information