|
Order Kazusa clone(s) from : |
| Product ID | ORK00831 |
|---|---|
| Accession No | AB037852 |
| Description | ZFP28 zinc finger protein, transcript variant 1 |
| Clone name | fj00022 |
| Vector information | |
| cDNA sequence | DNA sequence (4076 bp) Predicted protein sequence (891 aa) |
|
HaloTag ORF Clone |
FHC00831
|
| Flexi ORF Clone | FXC00831 |
| Source | Human fetal brain |
| Rouge ID |
mKIAA1431
by Kazusa Mouse cDNA Project
|
Length: 4076 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | No warning |
Integrity of 3' end
| Length of 3'UTR | 1398 bp |
|---|---|
| Genome contig ID | gi42406306f_61642129 |
| PolyA signal sequence (AATAAA,-16) |
+----*----+----*----+----*----+---- |
| Flanking genome sequence (117844 - 117893) |
----+----*----+----*----+----*----+----*----+----* |
Ensembl ContigView (Add our DAS server as a DAS source)
| Chr | f/r | start | end | exon | identity | class | |
|---|---|---|---|---|---|---|---|
|
| 19 | f | 61742129 | 61759971 | 8 | 99.2 | Perfect prediction |
Length: 891 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
|
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
|
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| BlastProDom | IPR007087 | 443 | 466 | PD000003 | Zinc finger |
| IPR007087 | 471 | 494 | PD000003 | Zinc finger | |
| IPR007087 | 499 | 523 | PD000003 | Zinc finger | |
| IPR007087 | 556 | 579 | PD000003 | Zinc finger | |
| IPR007087 | 584 | 607 | PD000003 | Zinc finger | |
| IPR007087 | 612 | 635 | PD000003 | Zinc finger | |
| IPR007087 | 640 | 663 | PD000003 | Zinc finger | |
| IPR007087 | 668 | 691 | PD000003 | Zinc finger | |
| IPR007087 | 696 | 719 | PD000003 | Zinc finger | |
| IPR007087 | 724 | 747 | PD000003 | Zinc finger | |
| IPR007087 | 752 | 775 | PD000003 | Zinc finger | |
| IPR007087 | 780 | 803 | PD000003 | Zinc finger | |
| IPR007087 | 808 | 831 | PD000003 | Zinc finger | |
| HMMPfam | IPR001909 | 126 | 166 | PF01352 | KRAB box |
| IPR001909 | 255 | 291 | PF01352 | KRAB box | |
| IPR007087 | 443 | 465 | PF00096 | Zinc finger | |
| IPR007087 | 471 | 493 | PF00096 | Zinc finger | |
| IPR007087 | 499 | 522 | PF00096 | Zinc finger | |
| IPR007087 | 528 | 550 | PF00096 | Zinc finger | |
| IPR007087 | 556 | 578 | PF00096 | Zinc finger | |
| IPR007087 | 584 | 606 | PF00096 | Zinc finger | |
| IPR007087 | 612 | 634 | PF00096 | Zinc finger | |
| IPR007087 | 640 | 662 | PF00096 | Zinc finger | |
| IPR007087 | 668 | 690 | PF00096 | Zinc finger | |
| IPR007087 | 696 | 718 | PF00096 | Zinc finger | |
| IPR007087 | 724 | 746 | PF00096 | Zinc finger | |
| IPR007087 | 752 | 774 | PF00096 | Zinc finger | |
| IPR007087 | 780 | 802 | PF00096 | Zinc finger | |
| IPR007087 | 808 | 830 | PF00096 | Zinc finger | |
| HMMSmart | IPR001909 | 126 | 186 | SM00349 | KRAB box |
| IPR001909 | 255 | 311 | SM00349 | KRAB box | |
| IPR015880 | 443 | 465 | SM00355 | Zinc finger | |
| IPR015880 | 471 | 493 | SM00355 | Zinc finger | |
| IPR015880 | 499 | 522 | SM00355 | Zinc finger | |
| IPR015880 | 528 | 550 | SM00355 | Zinc finger | |
| IPR015880 | 556 | 578 | SM00355 | Zinc finger | |
| IPR015880 | 584 | 606 | SM00355 | Zinc finger | |
| IPR015880 | 612 | 634 | SM00355 | Zinc finger | |
| IPR015880 | 640 | 662 | SM00355 | Zinc finger | |
| IPR015880 | 668 | 690 | SM00355 | Zinc finger | |
| IPR015880 | 696 | 718 | SM00355 | Zinc finger | |
| IPR015880 | 724 | 746 | SM00355 | Zinc finger | |
| IPR015880 | 752 | 774 | SM00355 | Zinc finger | |
| IPR015880 | 780 | 802 | SM00355 | Zinc finger | |
| IPR015880 | 808 | 830 | SM00355 | Zinc finger | |
| ProfileScan | IPR001909 | 126 | 197 | PS50805 | KRAB box |
| IPR001909 | 255 | 322 | PS50805 | KRAB box | |
| IPR007087 | 443 | 470 | PS50157 | Zinc finger | |
| IPR007087 | 471 | 498 | PS50157 | Zinc finger | |
| IPR007087 | 499 | 527 | PS50157 | Zinc finger | |
| IPR007087 | 528 | 555 | PS50157 | Zinc finger | |
| IPR007087 | 556 | 583 | PS50157 | Zinc finger | |
| IPR007087 | 584 | 611 | PS50157 | Zinc finger | |
| IPR007087 | 612 | 639 | PS50157 | Zinc finger | |
| IPR007087 | 640 | 667 | PS50157 | Zinc finger | |
| IPR007087 | 668 | 695 | PS50157 | Zinc finger | |
| IPR007087 | 696 | 723 | PS50157 | Zinc finger | |
| IPR007087 | 724 | 751 | PS50157 | Zinc finger | |
| IPR007087 | 752 | 779 | PS50157 | Zinc finger | |
| IPR007087 | 780 | 807 | PS50157 | Zinc finger | |
| IPR007087 | 808 | 835 | PS50157 | Zinc finger | |
| IPR007087 | 836 | 863 | PS50157 | Zinc finger | |
| ScanRegExp | IPR007087 | 445 | 465 | PS00028 | Zinc finger |
| IPR007087 | 473 | 493 | PS00028 | Zinc finger | |
| IPR007087 | 501 | 522 | PS00028 | Zinc finger | |
| IPR007087 | 530 | 550 | PS00028 | Zinc finger | |
| IPR007087 | 558 | 578 | PS00028 | Zinc finger | |
| IPR007087 | 586 | 606 | PS00028 | Zinc finger | |
| IPR007087 | 614 | 634 | PS00028 | Zinc finger | |
| IPR007087 | 642 | 662 | PS00028 | Zinc finger | |
| IPR007087 | 670 | 690 | PS00028 | Zinc finger | |
| IPR007087 | 698 | 718 | PS00028 | Zinc finger | |
| IPR007087 | 726 | 746 | PS00028 | Zinc finger | |
| IPR007087 | 754 | 774 | PS00028 | Zinc finger | |
| IPR007087 | 782 | 802 | PS00028 | Zinc finger | |
| IPR007087 | 810 | 830 | PS00028 | Zinc finger |
RT-PCR-ELISA
|
Experimental conditions| Primer_f | GCTGTGTGGAAGATCAAGGAG |
|---|---|
| Primer_r | CATCATAATCCCAGTGTTCCC |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 19
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | GCTGTGTGGAAGATCAAGGAG |
| Primer_r | CATCATAATCCCAGTGTTCCC |
| PCR product length | 136 bp |
| PCR conditions | 95 °C 15 sec 64 °C 60 sec 30 cycles |