KIAA1619

Product ID	ORK02043
Accession No	AB046839
Description	sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4G, transcript variant 1
Clone name	fj14720
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4094 bp) Predicted protein sequence (869 aa)
HaloTag ORF Clone	FHC02043
Flexi ORF Clone	FXC02043
Source	Human fetal brain
Rouge ID	mKIAA1619 by Kazusa Mouse cDNA Project

Length: 4094 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	1484 bp
Genome contig ID	gi89161187f_102622582
PolyA signal sequence (AATAAA,-22)	+--------+--------+----*----+---- CCTGCCGTTGGTTAATAAACACCCTTTTTCCCCAC
Flanking genome sequence (112782 - 112831)	----+--------+--------+--------+--------+----* AGCTATGACTAGTCTGCTTCATCTAGATGCGCCCACCCCTGATCACCAGG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	10	f	102722582	102735362	14	99.4	Perfect prediction

Length: 869 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
CAI10916	100.0	sema domain, im...	Homo sapiens
Q9NTN9	99.4	Semaphorin-4G; ...	Homo sapiens
XP_001109124	98.7	sema domain, im...	Macaca mulatta
XP_001109068	98.1	semaphorin 4G i...	Macaca mulatta
XP_001916730	93.7	sema domain, im...	Equus caballus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB051526	2.8e-53	41.5	KIAA1739
AB051532	1.5e-40	38.4	KIAA1745
AB002329	8.8e-21	31.5	KIAA0331
AB037789	8.6e-11	32.9	KIAA1368
AB040878	1e-10	29.5	KIAA1445

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR001627	82	515	PF01403	Semaphorin/CD100 antigen
HMMPfam	IPR002165	533	589	PF01437	Plexin
HMMSmart	IPR001627	82	515	SM00630	Semaphorin/CD100 antigen
	IPR003659	533	589	SM00423	Plexin/semaphorin/integrin
	IPR003599	600	682	SM00409	Immunoglobulin subtype
ProfileScan	IPR001627	61	531	PS51004	Semaphorin/CD100 antigen
ProfileScan	IPR007110	598	680	PS50835	Immunoglobulin-like

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	5	PPPQPVTLAPFGGLVSLGLP	24	SECONDARY	20
	2	28	WGRLWPLLLSILTATAVPGPSL	49	SECONDARY	22
	3	707	LYVLAIAALGGLCLILASSLLYV	729	PRIMARY	23

Primer_f	GTAGCTGAGAACGTGGAATCC
Primer_r	TGGTTGTAAGTGGGGGCATTC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	unigene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-