KIAA1021

Product ID	ORK04210
Accession No	AB028944
Description	ATPase, class VI, type 11A
Clone name	af10412
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (7611 bp) Predicted protein sequence (1102 aa)
Source	Human brain (amygdala)
Rouge ID	mKIAA1021 by Kazusa Mouse cDNA Project
Note	We replaced fg00592, former representative clones for KIAA1021 with af10412. (2005/08/06)

Length: 7611 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	Warning

Integrity of 3' end

Length of 3'UTR	4301 bp
Genome contig ID	gi51511729f_112387506
PolyA signal sequence (None)	+--------+--------+----*----+---- GCCAGCCTTCCGCCACGAGCCAGCTGGGAAGGGCC
Flanking genome sequence (200916 - 200965)	----+--------+--------+--------+--------+----* GCGGCCGCCTAAAGCCCCAGTCAACCCAGCCTGTGTCTGAGCAGACAGGG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	13	f	112487506	112588420	29	99.4	Perfect prediction

Length: 1102 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
P98196	99.9	Probable phosph...	Homo sapiens
ABY40831	99.3	ATPase, Class V...	Papio anubis
NP_115565	99.9	ATPase, class V...	Homo sapiens
XP_509744	99.7	ATPase, Class V...	Pan troglodytes
XP_001101635	99.3	similar to ATPa...	Macaca mulatta

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB023173	1.7e-93	57.9	KIAA0956
AB075819	8.6e-79	34.8	KIAA1939
AB032963	5.4e-74	36.2	KIAA1137
AB040920	1.1e-60	38.0	KIAA1487
AB018258	1.6e-58	32.1	KIAA0715

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001757	380	394	PR00119	ATPase
FPrintScan	IPR001757	791	810	PR00119	ATPase
HMMPfam	IPR005834	376	814	PF00702	Haloacid dehalogenase-like hydrolase
HMMTigr	IPR006539	13	1078	TIGR01652	Phospholipid-translocating P-type ATPase
	IPR001757	62	161	TIGR01494	ATPase
	IPR001757	622	672	TIGR01494	ATPase
	IPR001757	763	871	TIGR01494	ATPase
ScanRegExp	IPR001757	382	388	PS00154	ATPase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	49	FLVQLIIDTPTSPVTSGLPLFFV	71	PRIMARY	23
	2	261	MNAFLIVYLCILISKALINTVLK	283	PRIMARY	23
	3	315	TDFLAFMVLFNYIIPVSMYVTVE	337	PRIMARY	23
	4	852	QYFFYKNVCFIFPQFLYQFFCGF	874	SECONDARY	23
	5	884	YLTLYNISFTSLPILLYSLMEQH	906	SECONDARY	23
	6	934	IYWTLLGLFDALVFFFGAYFVFE	956	SECONDARY	23
	7	971	TFGTLVFTVMVFTVTLKLALDT	992	PRIMARY	22
	8	1000	HFVIWGSLLFYVVFSLLWGGVIW	1022	PRIMARY	23
	9	1034	FIQMLSSGPAWLAIVLLVTISLL	1056	PRIMARY	23

Primer_f	GCATTGGACACACACTACTGG
Primer_r	AACACGTAGTACATCCTCTGG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	UniGene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-