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Order Kazusa clone(s) from : |
| Product ID | ORK00509 |
|---|---|
| Accession No | AB002332 |
| Description | clock circadian regulator, transcript variant 2 |
| Clone name | hg01015 |
| Vector information | |
| cDNA sequence | DNA sequence (5715 bp) Predicted protein sequence (848 aa) |
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HaloTag ORF Clone |
FHC00509
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| Flexi ORF Clone | FXC00509 |
| Source | Human adult brain |
| Rouge ID |
mKIAA0334
by Kazusa Mouse cDNA Project
|
Length: 5715 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | No warning |
Length: 848 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| FPrintScan | IPR001067 | 51 | 66 | PR00785 | Nuclear translocator |
| IPR001067 | 102 | 125 | PR00785 | Nuclear translocator | |
| IPR001067 | 277 | 294 | PR00785 | Nuclear translocator | |
| HMMPfam | IPR001092 | 37 | 87 | PF00010 | Basic helix-loop-helix dimerisation region bHLH |
| IPR013767 | 111 | 178 | PF00989 | PAS fold | |
| IPR013655 | 288 | 381 | PF08447 | PAS fold-3 | |
| HMMSmart | IPR001092 | 42 | 92 | SM00353 | Basic helix-loop-helix dimerisation region bHLH |
| IPR000014 | 111 | 177 | SM00091 | PAS | |
| IPR000014 | 266 | 332 | SM00091 | PAS | |
| IPR001610 | 338 | 381 | SM00086 | PAC motif | |
| ProfileScan | IPR001092 | 35 | 87 | PS50888 | Basic helix-loop-helix dimerisation region bHLH |
| IPR000014 | 109 | 179 | PS50112 | PAS | |
| IPR000014 | 287 | 334 | PS50112 | PAS |
RT-PCR
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|---|
Experimental conditions| Primer_f | GGGCGGGATAGCTTTGCAACC |
|---|---|
| Primer_r | CGTAGTAGTGGAAGAGGTAGT |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 4
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | GGGCGGGATAGCTTTGCAACC |
| Primer_r | CGTAGTAGTGGAAGAGGTAGT |
| PCR product length | 96 bp |
| PCR conditions | 95 °C 15 sec 64 °C 60 sec 30 cycles |