KIAA1144

Product ID	ORK00186
Accession No	AB032970
Description	potassium voltage-gated channel, modifier subfamily S, member 2
Clone name	hj05565
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5027 bp) Predicted protein sequence (489 aa)
HaloTag ORF Clone	FHC00186
Flexi ORF Clone	FXC00186
Source	Human adult brain
Rouge ID	mKIAA1144 by Kazusa Mouse cDNA Project

Length: 5027 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	3438 bp
Genome contig ID	gi51511724f_99408621
PolyA signal sequence (None)	+--------+--------+----*----+---- ACACTACTGTGCAAGCATTGACACAGGCTGCACTG
Flanking genome sequence (105633 - 105682)	----+--------+--------+--------+--------+----* AACCATTATGTCTGATTGCTGCACTTTCATCTCTGTGCCTCGATTCGGGA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	8	f	99508621	99514252	2	99.0	Perfect prediction

Length: 489 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q9ULS6	9.6e-207	100.0	Potassium volta...	Homo sapiens
XP_001094800	2.9e-206	100.0	potassium volta...	Macaca mulatta
XP_001928892	1.9e-205	99.4	similar to Pota...	Sus scrofa
XP_592772	6.6e-205	99.0	similar to Pota...	Bos taurus
XP_539100	9.5e-205	96.5	similar to Pota...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB028967	7.2e-32	30.3	KIAA1044
AB037777	8.4e-05	26.2	KIAA1356
D79998	0.00023	32.6	KIAA0176

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR003971	34	46	PR01494	Kv9 voltage-gated K+ channel
	IPR003971	49	65	PR01494	Kv9 voltage-gated K+ channel
	IPR003971	72	85	PR01494	Kv9 voltage-gated K+ channel
	IPR003968	80	90	PR01491	Kv channel
	IPR003091	80	99	PR00169	Voltage-dependent potassium channel
	IPR003971	96	111	PR01494	Kv9 voltage-gated K+ channel
	IPR003971	119	131	PR01494	Kv9 voltage-gated K+ channel
	IPR003091	192	220	PR00169	Voltage-dependent potassium channel
	IPR003971	205	217	PR01494	Kv9 voltage-gated K+ channel
	IPR003091	241	264	PR00169	Voltage-dependent potassium channel
	IPR003091	267	287	PR00169	Voltage-dependent potassium channel
	IPR003091	311	337	PR00169	Voltage-dependent potassium channel
	IPR003968	316	324	PR01491	Kv channel
	IPR003968	340	354	PR01491	Kv channel
	IPR003091	340	363	PR00169	Voltage-dependent potassium channel
	IPR003091	370	392	PR00169	Voltage-dependent potassium channel
	IPR003091	399	425	PR00169	Voltage-dependent potassium channel
	IPR003971	409	427	PR01494	Kv9 voltage-gated K+ channel
	IPR003968	410	421	PR01491	Kv channel
HMMPfam	IPR003131	31	130	PF02214	K+ channel tetramerisation
HMMPfam	IPR005821	241	421	PF00520	Ion transport
HMMSmart	IPR000210	29	138	SM00225	BTB/POZ-like

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	197	LSRVFSILSILVVMGSIITMCLN	219	PRIMARY	23
	2	243	EHFGIAWFTFELVARFAVAPDFL	265	SECONDARY	23
	3	273	NLIDLMSIVPFYITLVVNLVVES	295	PRIMARY	23
	4	337	SYKEVGLLLLYLSVGISIFSVVA	359	SECONDARY	23
	5	367	NEGLATIPACWWWATVSMTTVGY	389	SECONDARY	23
	6	403	ASACILAGILVVVLPITLIFNKF	425	PRIMARY	23

Primer_f	GGTTGGGAAGTGACACGTCTG
Primer_r	ACCAATTACTACCTTACGAGC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	GGTTGGGAAGTGACACGTCTG
Primer_r	ACCAATTACTACCTTACGAGC
PCR product length	148 bp
PCR conditions	95 °C15 sec64 °C60 sec30 cycles