FLJ00393

Product ID	ORK05187
Accession No	AK090472
Clone name	sj05490
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4284 bp) Predicted protein sequence (547 aa)
Source	Human spleen

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Length: 547 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAC03453	100.0	FLJ00393 protei...	Homo sapiens
Q9UJP4	99.8	Kelch-like prot...	Homo sapiens
BAG52104	99.6	unnamed protein...	Homo sapiens
XP_001094118	99.3	similar to kelc...	Macaca mulatta
Q08DS0	96.5	Kelch-like prot...	Bos taurus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB007938	2.4e-161	99.6	KIAA0469
AB032955	6.6e-34	31.0	KIAA1129
AB051474	2.2e-31	30.8	KIAA1687
AB067508	6.8e-31	34.8	KIAA1921
AB040923	1e-29	29.1	KIAA1490

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR006651	461	474	PR00501	Kelch motif
FPrintScan	IPR006651	479	493	PR00501	Kelch motif
HMMPfam	IPR000210	1	83	PF00651	BTB/POZ
	IPR006652	225	271	PF01344	Kelch repeat
	IPR006652	274	319	PF01344	Kelch repeat
	IPR006652	321	359	PF01344	Kelch repeat
	IPR006652	361	405	PF01344	Kelch repeat
	IPR006652	409	449	PF01344	Kelch repeat
	NULL	451	497	PF07646	NULL
	IPR006652	451	497	PF01344	Kelch repeat
HMMSmart	IPR000210	1	83	SM00225	BTB/POZ
ProfileScan	IPR000210	1	53	PS50097	BTB/POZ

Primer_f
Primer_r
PCR conditions	test