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Order Kazusa clone(s) from : |
| Product ID | ORK00503 |
|---|---|
| Accession No | AB002315 |
| Description | apoptosis resistant E3 ubiquitin protein ligase 1 |
| Clone name | hg00276 |
| Vector information | |
| cDNA sequence | DNA sequence (5402 bp) Predicted protein sequence (826 aa) |
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HaloTag ORF Clone |
FHC00503
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| Flexi ORF Clone | FXC00503 |
| Source | Human adult brain |
| Rouge ID |
mKIAA0317
by Kazusa Mouse cDNA Project
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Length: 5402 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | No warning |
Length: 826 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| HMMPfam | IPR001298 | 71 | 158 | PF00630 | Filamin/ABP280 repeat |
| IPR000569 | 515 | 826 | PF00632 | HECT | |
| HMMSmart | IPR000569 | 484 | 826 | SM00119 | HECT |
| ProfileScan | IPR001298 | 55 | 161 | PS50194 | Filamin/ABP280 repeat |
| IPR000569 | 486 | 826 | PS50237 | HECT | |
| ScanRegExp | IPR000408 | 182 | 192 | PS00626 | Regulator of chromosome condensation |
RT-PCR
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Experimental conditions| Primer_f | CATGTCTGGTCTCCTTCAAAG |
|---|---|
| Primer_r | GTGGGGAAAAGGTACAATATG |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 14
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | CATGTCTGGTCTCCTTCAAAG |
| Primer_r | GTGGGGAAAAGGTACAATATG |
| PCR product length | 173 bp |
| PCR conditions | 95 °C 15 sec 62 °C 60 sec 30 cycles |