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Order Kazusa clone(s) from : |
| Product ID | ORK05759 |
|---|---|
| Accession No | AB051464 |
| Description | kelch-like family member 15 |
| Clone name | fh18965 |
| Vector information | |
| cDNA sequence | DNA sequence (5765 bp) Predicted protein sequence (521 aa) |
| Source | Human fetal brain |
Length: 5765 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | Warning | No warning |
Integrity of 3' end
| Length of 3'UTR | 4197 bp |
|---|---|
| Genome contig ID | gi89161218r_23811762 |
| PolyA signal sequence (AATAAA,-21) |
+----*----+----*----+----*----+---- |
| Flanking genome sequence (100000 - 99951) |
----+----*----+----*----+----*----+----*----+----* |
Length: 521 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| HMMPfam | IPR013069 | 2 | 45 | PF00651 | BTB/POZ |
| IPR011705 | 50 | 154 | PF07707 | BTB/Kelch-associated | |
| IPR006652 | 233 | 283 | PF01344 | Kelch repeat type 1 | |
| IPR006652 | 285 | 330 | PF01344 | Kelch repeat type 1 | |
| IPR006652 | 332 | 392 | PF01344 | Kelch repeat type 1 | |
| IPR006652 | 394 | 446 | PF01344 | Kelch repeat type 1 | |
| IPR006652 | 448 | 494 | PF01344 | Kelch repeat type 1 | |
| HMMSmart | IPR006652 | 245 | 296 | SM00612 | Kelch repeat type 1 |
| IPR006652 | 297 | 343 | SM00612 | Kelch repeat type 1 | |
| IPR006652 | 344 | 405 | SM00612 | Kelch repeat type 1 | |
| IPR006652 | 406 | 459 | SM00612 | Kelch repeat type 1 | |
| IPR006652 | 460 | 509 | SM00612 | Kelch repeat type 1 |
Prediction of transmembrane (TM) segments| Method | No. | N terminal | transmembrane region | C terminal | type | length | SOSUI2 | 1 | 25 | AMYVQLIEVVKFCCSFLLAKICL | 47 | PRIMARY | 23 |
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RT-PCR-ELISA
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Experimental conditions| Primer_f | TCTGTGTCCTCATTACTCTGC |
|---|---|
| Primer_r | CCTTTACTATGACTCGAGCAG |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. X
Experimental conditions| Panel name | CCR |
|---|---|
| Primer_f | TCTGTGTCCTCATTACTCTGC |
| Primer_r | CCTTTACTATGACTCGAGCAG |
| PCR product length | 137 bp |
| PCR conditions | 95 °C 15 sec 62 °C 60 sec 30 cycles |