KIAA1739

Product ID	ORK06763
Accession No	AB051526
Description	sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4C
Clone name	pj00464
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (3776 bp) Predicted protein sequence (963 aa)
Source	Human brain (hippocampus)
Rouge ID	mKIAA1739 by Kazusa Mouse cDNA Project

Length: 3776 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	884 bp
Genome contig ID	gi89161199r_96789206
PolyA signal sequence (AATAAA,-18)	+--------+--------+----*----+---- GTATTTTTATATTGACAAATAAAATGGAGAAAATG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAACGATTGCTCTGATGGGGCTAAGATGTTTGGGCTAAAGCCTTTCTGGG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	2	r	96889206	96896394	13	99.5	Perfect prediction

Length: 963 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
EAW71325	100.0	sema domain, im...	Homo sapiens
Q9C0C4	96.8	Semaphorin-4C; ...	Homo sapiens
AAQ89201	96.6	SEMA4C [Homo sa...	Homo sapiens
XP_001100467	94.1	semaphorin 4C [...	Macaca mulatta
XP_515634	84.2	semaphorin 4C [...	Pan troglodytes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB046839	1.1e-61	41.3	KIAA1619
AB051532	1.3e-54	40.5	KIAA1745
AB002329	1.6e-30	33.6	KIAA0331
AB058772	6.8e-21	35.6	KIAA1869
AB040912	1.7e-19	33.7	KIAA1479

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR001627	183	611	PF01403	Semaphorin/CD100 antigen
HMMPfam	IPR002165	629	681	PF01437	Plexin
HMMSmart	IPR001627	183	611	SM00630	Semaphorin/CD100 antigen
	IPR003659	629	681	SM00423	Plexin/semaphorin/integrin
	IPR003599	692	776	SM00409	Immunoglobulin subtype
ProfileScan	IPR001627	160	627	PS51004	Semaphorin/CD100 antigen
ProfileScan	IPR007110	686	767	PS50835	Immunoglobulin-like

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	25	HSRLCLLLCWTLIEAVGSR	43	SECONDARY	19
	2	730	PGSFLYDARLQALVVMAA	747	SECONDARY	18
	3	765	RLAAEGYLVAVVAGPSVTLEAR	786	SECONDARY	22
	4	791	NLGLVWLAVVALGAVCLVLLLLV	813	PRIMARY	23

Primer_f	ATGCTGGCCTTCTTGTGGATG
Primer_r	CACACTCTCAGGTACATAGGC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	unigene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-