KIAA1869

Product ID	ORK00937
Accession No	AB058772
Description	sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6C, transcript variant 2
Clone name	hh01800
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5261 bp) Predicted protein sequence (935 aa)
HaloTag ORF Clone	FHC00937
Flexi ORF Clone	FXC00937
Source	Human adult brain
Rouge ID	mKIAA1869 by Kazusa Mouse cDNA Project

Length: 5261 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	776 bp
Genome contig ID	gi89161185r_149270808
PolyA signal sequence (AATAAA,-26)	+--------+--------+----*----+---- TTTTTGAGAAATAAAATGAAGCTGCAGTGTACGTT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ACTTGAAGTTCCACATCAGAAAATCTTGTCCTTAGGAGAATTTGTCTGGC

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	1	r	149370808	149385711	19	100.0	Perfect prediction

Length: 935 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
CAI16378	100.0	sema domain, tr...	Homo sapiens
BAB20670	99.9	semaphorin Y [H...	Homo sapiens
Q9H3T2	99.9	Semaphorin-6C; ...	Homo sapiens
XP_001104082	97.8	semaphorin Y is...	Macaca mulatta
XP_001929695	89.9	similar to Sema...	Sus scrofa

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB040912	3.8e-51	45.3	KIAA1479
AB037789	1.6e-43	38.6	KIAA1368
AB040878	5.7e-17	36.2	KIAA1445
AB002329	8e-14	30.8	KIAA0331
AB051526	1.2e-13	35.6	KIAA1739

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR001627	69	496	PF01403	Semaphorin/CD100 antigen
HMMPfam	IPR002165	523	544	PF01437	Plexin
HMMSmart	IPR001627	66	494	SM00630	Semaphorin/CD100 antigen
ProfileScan	IPR001627	35	521	PS51004	Semaphorin/CD100 antigen

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	7	PRAPHFMPLLLLLLLLSLPHTQ	28	SECONDARY	22
SOSUI2	2	610	LLLASVAAAFALGASVSGLLVSC	632	PRIMARY	23

Primer_f	TCGCTCTCTGCCTTCATCTGG
Primer_r	CACCGTTAATTCGCTCGTCTC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	CTCGCTCTCTGCCTTCATCTG
Primer_r	CTTCCCCAGCCTCAACTAAAC
PCR product length	95 bp
PCR conditions	15 °C66 sec60 °C30 sec170 cycles