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Order Kazusa clone(s) from : |
| Product ID | ORK05758 |
|---|---|
| Accession No | AB037730 |
| Description | kelch-like family member 13 |
| Clone name | fh10381 |
| Vector information | |
| cDNA sequence | DNA sequence (5331 bp) Predicted protein sequence (639 aa) |
| Source | Human fetal brain |
| Rouge ID |
mKIAA1309
by Kazusa Mouse cDNA Project
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Length: 5331 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | No warning |
Integrity of 3' end
| Length of 3'UTR | 1094 bp |
|---|---|
| Genome contig ID | gi89161218r_116815805 |
| PolyA signal sequence (AATAAA,-19) |
+----*----+----*----+----*----+---- |
| Flanking genome sequence (100000 - 99951) |
----+----*----+----*----+----*----+----*----+----* |
Length: 639 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| HMMPfam | IPR013069 | 66 | 175 | PF00651 | BTB/POZ |
| IPR011705 | 180 | 281 | PF07707 | BTB/Kelch-associated | |
| IPR006652 | 313 | 360 | PF01344 | Kelch repeat type 1 | |
| IPR006652 | 362 | 412 | PF01344 | Kelch repeat type 1 | |
| IPR006652 | 414 | 459 | PF01344 | Kelch repeat type 1 | |
| IPR006652 | 461 | 506 | PF01344 | Kelch repeat type 1 | |
| IPR006652 | 508 | 558 | PF01344 | Kelch repeat type 1 | |
| IPR006652 | 562 | 607 | PF01344 | Kelch repeat type 1 | |
| HMMSmart | IPR000210 | 76 | 175 | SM00225 | BTB/POZ-like |
| IPR006652 | 325 | 373 | SM00612 | Kelch repeat type 1 | |
| IPR006652 | 374 | 425 | SM00612 | Kelch repeat type 1 | |
| IPR006652 | 426 | 472 | SM00612 | Kelch repeat type 1 | |
| IPR006652 | 473 | 519 | SM00612 | Kelch repeat type 1 | |
| IPR006652 | 520 | 571 | SM00612 | Kelch repeat type 1 | |
| IPR006652 | 572 | 620 | SM00612 | Kelch repeat type 1 | |
| ProfileScan | IPR000210 | 76 | 145 | PS50097 | BTB/POZ-like |
RT-PCR-ELISA
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Experimental conditions| Primer_f | TGACTGTGTGGGTGCATTGAG |
|---|---|
| Primer_r | TAAGTAGAGGGTTCATGCTGC |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 9
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | TGACTGTGTGGGTGCATTGAG |
| Primer_r | TAAGTAGAGGGTTCATGCTGC |
| PCR product length | 104 bp |
| PCR conditions | 95 °C 15 sec 64 °C 60 sec 30 cycles |